منابع مشابه
FAST-NMR: functional annotation screening technology using NMR spectroscopy.
An abundance of protein structures emerging from structural genomics and the Protein Structure Initiative (PSI) are not amenable to ready functional assignment because of a lack of sequence and structural homology to proteins of known function. We describe a high-throughput NMR methodology (FAST-NMR) to annotate the biological function of novel proteins through the structural and sequence analy...
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Considerable excitement has been aroused by recent new methods for speeding up multidimensional NMR experiments by radically modifying the normal time-domain sampling protocols. These new schemes include the filter diagonalization method, GFT-NMR, the single-scan two-dimensional technique, Hadamard spectroscopy, and a proposal based on projection-reconstruction of three-dimensional spectra. All...
متن کاملFast multi-dimensional NMR of proteins.
Three-dimensional HNCO and HNCA subspectra from a small protein (agitoxin, 4 kDa, enriched in carbon-13 and nitrogen-15), have been obtained by direct frequency-domain excitation of selected carbon and nitrogen sites. This new technique applies an array of several simultaneous soft radiofrequency spin-inversion pulses, encoded (on or off) according to nested Hadamard matrices, and the resulting...
متن کاملFast Multidimensional NMR Spectroscopy by the Projection – Reconstruction Technique
16 Spectroscopy 19(10) October 2004 Great strides were made in high-resolution nuclear magnetic resonance (NMR) spectroscopy with the introduction of two-dimensional (2-D) Fourier transformation (1, 2), which spreads the important information into two frequency dimensions. For the first time, spectroscopists were presented with a simple graphical picture of the correlations among different nucl...
متن کاملThe Radon Transform: A New Scheme for Fast Multidimensional NMR
Multidimensional nuclear magnetic resonance (NMR) spectra are used widely for structure elucidation of molecules of biochemical interest, particularly proteins, but the existing methodology is severely hampered by the long duration of the measurement, often many days. Here, we show that the inverse Radon transform can be used to reconstruct such a spectrum from a small number of different plane...
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ژورنال
عنوان ژورنال: EPJ Web of Conferences
سال: 2012
ISSN: 2100-014X
DOI: 10.1051/epjconf/20123005002